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Figure 8. Gene expression profiles between PCL/1Cu + NIR group and ZL group. (A) Volcano map of differentially expressed genes; (B) Heatmap for global gene expression; (C) GO enrichment analysis of the top 10 different genes; (D) KEGG pathway analysis of up-regulated genes; (F) KEGG pathway analysis of down-regulated genes; (E, G) Western blot of 𝛽-catenin and <t>WNT-3A</t> in hBMSCs treated with different methods for 10 days; (H) Schematic diagram of Zn2+ action on Wnt/𝛽-catenin and NF-𝜅B signaling pathway. n = 3.
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Figure 8. Gene expression profiles between PCL/1Cu + NIR group and ZL group. (A) Volcano map of differentially expressed genes; (B) Heatmap for global gene expression; (C) GO enrichment analysis of the top 10 different genes; (D) KEGG pathway analysis of up-regulated genes; (F) KEGG pathway analysis of down-regulated genes; (E, G) Western blot of 𝛽-catenin and WNT-3A in hBMSCs treated with different methods for 10 days; (H) Schematic diagram of Zn2+ action on Wnt/𝛽-catenin and NF-𝜅B signaling pathway. n = 3.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Photothermal Coating on Zinc Alloy for Controlled Biodegradation and Improved Osseointegration.

doi: 10.1002/advs.202409051

Figure Lengend Snippet: Figure 8. Gene expression profiles between PCL/1Cu + NIR group and ZL group. (A) Volcano map of differentially expressed genes; (B) Heatmap for global gene expression; (C) GO enrichment analysis of the top 10 different genes; (D) KEGG pathway analysis of up-regulated genes; (F) KEGG pathway analysis of down-regulated genes; (E, G) Western blot of 𝛽-catenin and WNT-3A in hBMSCs treated with different methods for 10 days; (H) Schematic diagram of Zn2+ action on Wnt/𝛽-catenin and NF-𝜅B signaling pathway. n = 3.

Article Snippet: Membranes containing nuclear extracts from hBMSCs were incubated overnight at 4 °C with primary antibodies specific to nuclear markers β-catenin, WNT-3A, p65, phospho-p65 (p-p65), IκBα, phosphorylated IκBα (p-IκBα), and GAPDH (Proteintech, China).

Techniques: Gene Expression, Western Blot